RESUMO
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Assuntos
Humanos , Masculino , Adulto Jovem , Hipersensibilidade Alimentar/diagnóstico , Produtos Pesqueiros/efeitos adversos , Eletroforese em Gel de Poliacrilamida , Western Blotting , Testes CutâneosRESUMO
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Assuntos
Humanos , Animais , Feminino , Adulto , Alérgenos/imunologia , Conjuntivite Alérgica/diagnóstico , Conjuntivite Alérgica/imunologia , Pediculus/imunologia , Rinite Alérgica/diagnóstico , Rinite Alérgica/imunologia , Testes Sorológicos , Testes CutâneosRESUMO
No disponible
Assuntos
Humanos , Feminino , Adulto , Alérgenos/efeitos adversos , Araceae/efeitos adversos , Hipersensibilidade/etiologia , Flores , Folhas de Planta , Caules de PlantaRESUMO
No disponible
Assuntos
Feminino , Humanos , Pessoa de Meia-Idade , Hipersensibilidade a Noz/diagnóstico , Hipersensibilidade a Noz/imunologia , Hipersensibilidade Imediata/imunologia , Testes Cutâneos/métodos , Hipersensibilidade a Noz/terapia , Hipersensibilidade Alimentar , Eletroforese em Gel de Poliacrilamida/métodos , Western Blotting/métodosRESUMO
BACKGROUND: Our group previously found that up to 7% of amateur anglers in Caceres, Spain may be allergic to the larvae of Protophormia terraenovae (order Diptera, family Calliphoridae) used as live bait for fishing. OBJECTIVE: To identify the pattern of major allergens in P terraenovae and other species of Calliphoridae. MATERIALS AND METHODS: Extracts of P terraenovae, Calliphora vomitoria, Lucilia sericata and Lumbricus terrestris were characterized using sodium dodecyl sulfate polyacrylamide gel electrophoresis and IgE-immunoblotting techniques in individual sera from 24 patients with a positive skin test result and/or specific IgE determination (enzyme-linked immunosorbent assay [ELISA]) to P terraenovae. ELISA and IgE-immunoblotting inhibition studies were also performed to identify potential cross-reactive allergens between these species. RESULTS: IgE-immunoblotting with P terraenovae showed a band of 15.3 kDa recognized by 15 patients, in addition to 2 further allergens of 22.8 kDa and 69 kDa. For C vomitoria, 5 bands of 73, 46, 40, 28, and 14 kDa were observed. For L sericata, 2 major allergens of 73 kDa and 14 kDa were observed. In the case of L terrestris, IgE from 13 patients recognized 1 allergen of around 15.5 kDa. IgE-immunoblotting and ELISA inhibition revealed the presence of cross-reactivity, mainly between L terrestris and P terraenovae. CONCLUSIONS: P terraenovae appears to have species-specific allergens and allergens shared with C vomitoria and L sericata. Striking immunological cross-reactivity was observed between P terraenovae and L terrestris. An allergen of 15-16 kDa could be involved in this phenomenon.
Assuntos
Alérgenos/imunologia , Dípteros/imunologia , Hipersensibilidade/imunologia , Larva/imunologia , Oligoquetos/imunologia , Animais , Western Blotting , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , EspanhaRESUMO
Antecedentes: La larva de Protophormia terraenovae, utilizada como cebo vivo para la pesca, es capaz de producir reacciones alérgicas en el 7% de la población de pescadores de agua dulce de Cáceres, según las observaciones previas de nuestro grupo. Objetivo: Identificar el patrón de alérgenos en P. terranovae y otros Califóridos. Materiales y métodos: Los extractos de P. terraenovae, Calliphora vomitoria, Lucilia sericata y Lumbricus terrestris se sometieron a técnicas de SDS-PAGE e IgE-immunoblotting utilizando sueros individuales de 24 pacientes sensibilizados a P. terraenovae. Se realizaron también técnicas de ELISA e IgE-immunoblotting inhibition para la identificación de posibles alérgenos comunes entre dichas especies. Resultados: 15 pacientes reconocieron una banda entorno a los 15.3 kDa frente al extracto de P. terraenovae, además de otros 2 alérgenos de 22.8 and 69kDa. Con C. vomitoria, se observaron 5 bandas de 73, 46, 40, 28 y 14 kDa. Con L. sericata se observaron 2 alérgenos mayores de 73 y 14 kDa. Usando L terrestris, 13 pacientes reconocieron un alérgeno de unos 15.5 kDa. Los estudios de inhibición IgE demostraron la presencia de reactividad cruzada inmunológica principalmente entre L. terrestris y P. terraenovae. Conclusiones: La larva de P. terraenovae parece tener alérgenos especie-específi cos y alérgenos compartidos con C. vomitoria y L. sericata. Se ha observado una importante reactividad cruzada inmunológica entre P. terraenovae y L. terrestris. Un alérgeno entre los 15-16 kDa podría ser uno de los responsables de este fenómeno (AU)
Background: Our group previously found that up to 7% of amateur anglers in Caceres, Spain may be allergic to the larvae of Protophormia terraenovae (order Diptera, family Calliphoridae) used as live bait for fishing. Objective: To identify the pattern of major allergens in P terraenovae and other species of Calliphoridae. Materials and Methods: Extracts of P terraenovae, Calliphora vomitoria, Lucilia sericata and Lumbricus terrestris were characterized using sodium dodecyl sulfate polyacrylamide gel electrophoresis and IgE-immunoblotting techniques in individual sera from 24 patients with a positive skin test result and/or specific IgE determination (enzyme-linked immunosorbent assay [ELISA]) to P terraenovae. ELISA and IgE-immunoblotting inhibition studies were also performed to identify potential cross-reactive allergens between these species. Results: IgE-immunoblotting with P terraenovae showed a band of 15.3 kDa recognized by 15 patients, in addition to 2 further allergens of 22.8 kDa and 69 kDa. For C vomitoria, 5 bands of 73, 46, 40, 28, and 14 kDa were observed. For L sericata, 2 major allergens of 73 kDa and 14 kDa were observed. In the case of L terrestris, IgE from 13 patients recognized 1 allergen of around 15.5 kDa. IgE-immunoblotting and ELISA inhibition revealed the presence of cross-reactivity, mainly between L terrestris and P terraenovae. Conclusions: P terraenovae appears to have species-specific allergens and allergens shared with C vomitoria and L sericata. Striking immunological cross-reactivity was observed between P terraenovae and L terrestris. An allergen of 15-16 kDa could be involved in this phenomenon (AU)
Assuntos
Animais , Oligoquetos/imunologia , Alérgenos/análise , Alérgenos/química , Alérgenos , Dessensibilização Imunológica , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Peixes/imunologia , Alérgenos/imunologia , Eletroforese em Gel de Poliacrilamida/métodos , Eletroforese em Gel de Poliacrilamida/normas , Eletroforese em Gel de Poliacrilamida , Western Blotting/métodos , Western Blotting/normas , Western Blotting , Ensaio de Imunoadsorção Enzimática/instrumentação , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção EnzimáticaRESUMO
No disponible